Tritium Labeling of Hexachlorocyclohexane Isomers by Gas Exposure Method. II

Title The Stereochemistry of Tritium Labeling of Hexachlorocyclohexane Isomers

A general method of tritium labeling utilizing microwave discharge activation of tritium gas. Methodology and application to biological compounds.

A method of general tritium labeling is described which is applicable to a broad range of organic compounds of biological interest, including amino acids, peptides, and proteins. The method is rapid, inexpensive, and nondestructive, thereby making the purification of labeled material relatively uncomplicated. The reaction apparatus consists of a closed system in which tritium gas at a pressure ...

Environmental Risk and Bioremediation of Hexachlorocyclohexane Isomers

Pesticide residues in Indian agricultural soils due to extensive and sometimes indiscriminate use still pose a serious problem for the production and export of agricultural goods. Residues of pestcides including HCH (hexachlorocyclohexane) have even been detected in popular soft drinks such as CocaCola and Pepsi. In addition, at pesticide production sites, soils of the surrounding area are ofte...

Critical Review Differential Toxicity and Environmental Fates of Hexachlorocyclohexane Isomers

The differential environmental fates and toxicities of the various hexachlorocyclohexane (HCH) isomers including lindane and isomers in the technical mixture will be the focus of this review. HCHs are one of the most widely used and most readily detected organochlorine pesticides in environmental samples. The relatively high volatility of HCH has led to global transport, even into formerly pris...

Tritium labeling of antisense oligonucleotides by exchange with tritiated water.

We describe a simple, efficient, procedure for labeling oligonucleotides to high specific activity (< 1 x 10(8) cpm/mumol) by hydrogen exchange with tritiated water at the C8 positions of purines in the presence of beta-mercaptoethanol, an effective radical scavenger. Approximately 90% of the starting material is recovered as intact, labeled oligonucleotide. The radiolabeled compounds are stabl...